Inhibition of proinflammatory mediators by coumaroyl lupendioic acid, a new lupane-type triterpene from Careya arborea , on inflammation-induced animal model

This study aimed at the isolation and evaluation of the anti-inflammatory effect of coumaroyl lupendioic acid, a new lupane-type triterpene from Careya arborea stem bark. Coumaroyl lupendioic acid was investigated in carrageenan induced inflammation and its effect was also compared with betulinic acid (BA). The effect of CLA on the production of NO, MPO, PGE2, TNF-α, IL-1β and IL-6 were assessed. In addition, the histopathology and immunohistochemistry (NF-ҡB, COX-2 and TNF-α protein expression) in paw tissues were also carried out. The study gives an insight into the molecular mechanisms of coumaroyl lupendioic acid and suggests that the down-regulations of proinflammatory mediators.

Promising anitidiabetic potential of Cuscuta reflexa leaves methanol extract in alloxan-induced diabetic rats

The antidiabetic activity of C. reflexa leaves methanol extract (CRME) was evaluated using alloxan-induced diabetes in Wistar albino rats. The effect of CRME was investigated by evaluating the blood glucose concentrations, HbA_1C, insulin, lipid profile and liver function test. Further, the protective potentials of CRME were studied by histopathology of the pancreas, liver, and kidney tissues from experimental rats. Our research exertion clearly demonstrates that CRME can be explored as a substantial antidiabetic and organ protective agent in the management of diabetes.

Ethnopharmacological Inspections of both Methanolic and Ethanolic Extract of Oroxylum indicum in Rat Models: A Promising Natural Gift

The stem bark of Oroxylum indicum (O. indicum) was aimed at testing for anti-inflammatory, antiulcerative, antihyperglycemic, and antidyslipidemic activities. Liver enzyme concentration (SGPT, SGOT) had also been assessed. This study remarked that O. indicum has anti-inflammatory, antiulcer, antidiabetic, and antidyslipidemic potentials but has no ameliorative effect on liver enzyme.

Pharmacological investigation on the stem bark of Careya arborea as a potential antiinflammatory agent in CFA induced chronic inflammation
 

Evaluation of gastroprotective and wound healing potential of phyllanthus niruri L. (Euphorbiaceae) leaves in experimental rats
 

Coumaroyl lupendioic acid attenuates oxidative stress in adjuvant induced arthritis
 

Inhibitory effect of coumaroyl lupendioic acid on inflammation mediator generation in CFA induced arthritis
Inhibitory effect of coumaroyl lupendioic acid on inflammation mediator generation in CFA induced arthritis
The present study was focused on investigating the anti-arthritic effect of coumaroyl lupendioic acid.  Arthritis was induced by injecting 0.1 ml of Complete Freund’s Adjuvant (5 mg/ml of heat killed Mycobacterium tuberculosis) into the subplantar region of the left hind paw. The effect of coumaroyl lupendioic acid (CLA) on the production PGE₂, NO, MPO, NF-κB, TNF-α, IL-1β, and IL-6 on serum level as well as inflamed paw tissue were also assessed. Coumaroyl lupendioic acid inhibits inflammation may be through the suppression of the cascade of proinflammatory mediators via the down-regulation of NF-ҡB.

Phytochemical analysis and investigation of antiinflammatory and anti-ulcer activity of Terminalia Bellirica Leaves Extract

The present study was undertaken to provide the rationale for the use of Terminalia bellirica as an anti-inflammatory agent using carrageenan-induced inflammation and antiulcer agent using ethanol-acid induced gastric mucosal injury model in the Swiss albino rats.The study clearly shows that methanolic extract of T. bellirica leaf possesses potent anti-inflammatory activity and promotes ulcer protection as ascertained by regeneration of mucosal layer and substantial prevention of the formation of hemorrhage and edema.

Inhibition of α-glucosidase by new prenylated flavonoids from Euphorbia hirta L. herb
To isolate and characterize the constituents of Euphorbia hirta and evaluate their in-vitro α-glucosidase inhibitory activity. The study is also aimed at describing structural activity relationship, type of α-glucosidase inhibition and in-vivo potential to regulate post prandial hyperglycemia in Wistar rats.

α-Glucosidase inhibitory activity and sucrose tolerance test demonstrated by the prenylated flavonoids present in E. hirta provide credence to the ethnomedicinal use of the plant in the management of diabetes in folk medicine.

Evaluation of the anti-inflammatory effect of Careya arborea in CFA induced chronic inflammation
 

The objective of the study was to explore the anti-inflammatory effect of methanolic extract of stem bark of Careya arborea Roxb, a plant used locally in India for various painful inflammatory conditions; using chronic inflammatory model of Complete. Inflammation was induced by injecting 0.1 ml of CFA containing 5 mg/ml of heat killed Mycobacterium tuberculosis into the sub plantar region of the left hind paw. Treatment with the extract and standard was started on the day of induction of inflamogens and continued up to 28 days. The effect of MECA on the production of nitric oxide, myeloperoxidase, gamma glutamyl transferase, malondialdehyde and C-reactive protein were determined. Our findings suggest that methanolic extract of C. arborea stem bark exhibited potent anti-inflammatory effects in CFA induced chronic inflammation.

Complement activation in obesity, insulin resistance, and type 2 diabetes mellitus
The focus of this review is to summarize the role of the activation of complement system and the initiation and progression of metabolic disorders including obesity, insulin resistance and diabetes mellitus. In addition, we briefly describe the interaction of the activation of the complement system with diabetic complications such as diabetic retinopathy, nephropathy and neuropathy, highlighting that targeting complement system therapeutics could be one of possible routes to slow down those aforementioned diabetic complications.

Analysis of prescription pattern of antibiotic drugs on patients suffering from ENT infection within Dhaka Metropolis, Bangladesh

This survey was designed to assess and evaluate the prescription pattern of antibiotic drugs on patients suffering from ENT infection within Dhaka Metropolis, Bangladesh. Methods: A cross sectional, observational and prospective study was conducted from January to June 2015 in the out-patients (OPD) and in-patients (IPD) of ENT department at different general and specialized government and private hospitals (Dhaka Medical College and Hospital, Sir Solimullah Medical College Mitford Hospital, Holy Family Red Crescent Medical College and Hospital, Dhaka Community Medical College and Hospital) within Dhaka city

Prescribing more than one antibiotics was commonly encountered in this study indicating the occurrence of polypharmacy. 

Effect of Dietary Magnesium and Calcium on Reducing the Risk of Atherosclerosis in High-Fat Diet Fed Rats
 

Wister Albino rats were divided into different groups (n = 4) based on their diets (Normal diet, High-Fat Diet (HFD)/atherogenic diet, HFD+Mg/Ca salts). HFD induced atherogenic rats were treated with either single or combined doses of MgCO3 (200, 400 and 600mg/kg/day) and CaCO3 (500, 1000 and 1500mg/kg/day) salts in diet for 12 weeks. The extent of atherosclerosis in experimental animals were analyzed by measuring body weight, systolic blood pressure (SBP) and serum lipid levels (cholesterol, triglyceride, LDL, HDL). Histopathology of the aorta, kidney and liver of atherogenic model was performed. Our present investigation indicates that a combination of high magnesium and moderate to high calcium salts in diet shows potential response against atherosclerosis.

Evaluation of anti-inflammatory and gastric anti-ulcer activity of Phyllanthus niruri L.(Euphorbiaceae) leaves in experimental rats
Inflammation was induced by injecting 0.1 ml carrageenan (1% w/v) into the left hind paw. Paw tissues from the different groups were examined for inflammatory cell infiltration. On the other hand, antiulcer activity of methanolic extract of P. niruri leaves at the doses of 100, 200 and 400 mg/kg, p.o. were examined against ethanol-acid induced gastric mucosal injury in the Swiss albino rats. The rats were dissected and the stomachs were macroscopically examined to identify hemorrhagic lesions in the glandular mucosa. The investigation suggests that methanolic extract of P. niruri leaf possess anti-inflammatory activity and promotes ulcer protection.

Wound healing effect of Euphorbia hirta linn. (Euphorbiaceae) in alloxan induced diabetic rats

A single injection of alloxan monohydrate (120 mg/kg, i.p.) prepared in normal saline was administered to produce diabetes in rats, after overnight fasting. For analyzing the rate of contraction of wound, excision wounds sized 4.90cm² and of 2 mm depth were used. The diabetic wound healing mechanism was studied by measuring the plasma level of glucose, malondialdehyde (MDA) and nitric oxide (NO) in both control and treated groups. For the confirmation of activity, histopathology of the wounds tissues from excision wound model was performed. The present study demonstrates that E. hirta whole plant extract promotes healing of wounds more significantly as compared to diabetic control rats, where healing is otherwise delayed.

Inhibitory effect of Careya arborea on inflammatory biomarkers in carrageenan-induced inflammation

The present study investigates the anti-inflammatory potential of the methanol extract of C. arborea stem bark and further assesses its possible mechanism on the modulation of inflammatory biomarkers. Materials and methods: Anti-inflammatory activity of C. arborea methanol extract (CAME) was evaluated (100 and 200 mg/kg, p.o) using indomethacin (10 mg/kg, p.o) as the standard drug in Wistar albino rats. Inflammation was induced by injecting 0.1 ml carrageenan (1% w/v) into the left hind paw. The anti-inflammatory mechanism was studied by measuring malondialdehyde (MDA), C-reactive protein (CRP), nitric oxide (NO), myeloperoxidase (MPO), TNF-α, and IL-1β levels in both control and treated groups. A protocol has also been established to quantify quercetin and betulinic acid content in CAME using HPTLC fingerprint. 

SAARC Scholarship to do PhD in Pharmacology at Jamia Hamdard, New Delhi, India

Best Presentation Award; Conference: 20^th International Conference on Alternative Healthcare and Herbal Remedies, New York, USA 2018.

• Research Skills: Measurement of biomarkers - Immunohistochemistry - Biosafety and animal cell culture - Extraction techniques - Animal models - Biopharmaceutics

• Job task analysis - Curriculum design - Assessment and Evaluation - Organizational administration

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• Teaching- Research - Planning – Laboratory analysis - Data analysis - Presentation